厚皮甜瓜新品种红宝玉的选育

红宝玉是以10R15 为母本、10R8 为父本选育而成的中熟厚皮甜瓜一代杂种。植株长势稳健，抗病抗逆性强。全生育期95～110 d（天），果实发育期30～35 d（天）。果实短椭圆形，成熟后果面乳白色，单果质量约1.5 kg，果肉橘红色，肉厚3.5～4.0 cm，腔小不发酵，中心可溶性固形物含量17.5%，肉质紧脆，不脱蒂，耐贮耐运。平均每667 m²产量3 600 kg 左右。适宜在山东、河北、陕西、安徽等地春季保护地设施栽培。     

Up-regulation of miR-181a attenuates releases of CSE-induced pro-inflammatory factors and expression of collagen IV,fibronectin and α-SMA in human bronchial epithelial cells

AIM: To investigate the effect and potential mechanism of microRNA-181a (miR-181a) on cigarette smoke extract (CSE)-induced the productions of pro-inflammatory factors and the expression of collagen IV, fibronectin and α-smooth muscle actin (α-SMA) in human bronchial epithelial cells (HBECs). METHODS: CSE-induced miR-181a expression was detected by RT-qPCR in the HBECs. After tansfected with miR-181a mimic, the releases of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6 and transforming growth factor-β1 (TGF-β1) were measured by ELISA, the protein expression of collagen IV, fibronectin and α-SMA was determined by Western blot. The activation of NF-κB/TGF-β1/Smad3 pathway was also evaluated by Western blot. RESULTS: CSE increased the levels of TNF-α, IL-1β, IL-6 and TGF-β1 and the expression of collagen IV, fibronectin and α-SMA, and decreased the expression of miR-181a in the HBECs (P<0.05). However, transfected with miR-181a mimic partially prevented the releases of TNF-α, IL-1β, IL-6 and TGF-β1, and inhibited the expression of collagen IV, fibronectin and α-SMA (P<0.05). Additionally, the activation of NF-κB/TGF-β1/Smad3 evoked by CSE was attenuated after transfected with miR-181a mimic. CONCLUSION: Up-regulation of miR-181a prevents the releases of CSE-induced pro-inflammatory factors and expression of collagen IV, fibronectin and α-SMA in the HBECs, and its mechanism may be related to the inhibition of NF-κB/TGF-β1/Smad3 pathway.     

基于Lucid多途径检索的油茶害虫诊断APP开发与实现

油茶是亚热带地区重要的木本油料作物,近年来随着种植面积的不断扩大和集约化经营,病虫害问题日益严重,对油茶的产量和品质造成不同程度的影响。本研究通过野外调查、文献查阅、种类鉴定等工作,整理了21种油茶主要害虫的形态特征、生物学习性、为害特点、防治措施等信息,并拍摄相应害虫及为害状的高清照片1 600张。采用Lucid智能诊断系统,构建Fact Sheet Fusion基础信息数据库,并针对多途径检索方式提取害虫"为害方式"、"为害部位"、"为害高峰期"、"形态特征"等4个1级特征组,以及10个2级特征和18个3级特征,共组成102个特征状态,构建了油茶害虫的快速诊断系统。在此基础上,转换得到基于Android系统的手机应用APP,为林农和森防一线工作者对油茶害虫快速识别和防治提供便捷服务,从而促进油茶产业持续健康发展。     

Effect of miR-29b-mediated TGF-β/Smad signaling pathway on progression of hepatic fibrosis in rats

AIM: To investigate the role of microRNA-29b (miR-29b)-mediated TGF-β/Smad signaling pathway in the activation of hepatic stellate cells (HSC) and its effect on the progression of hepatic fibrosis in rats.METHODS: Hepatic liver fibrosis rat model was established, and its HSC were isolated. Normal rat HSC were also obtained and identified in vitro. RT-qPCR and Western blot were used to detect the alterations of miR-29b, TGF-β/Smad signaling pathway-related proteins and liver fibrosis marker proteins in the acquired cells. Finally, the direct targeting binding of miR-29b to TGF-β1 was identified by dual-luciferase reporter assay system.RESULTS: With the activation of HSC, the expression of miR-29b gradually decreased (P<0.01), while the expression of collagen type I and α-smooth muscle actin gradually increased (P<0.01). At the same time, the expression of Smad2/3/4 was significantly increased, and the expression of Smad7 was significantly decreased (P<0.01). Dual-luciferase reporter assay showed that miR-29b bound directly to "UCUCUCCGU" in the 3'UTR of TGF-β1, indicating that TGF-β1 was a downstream target gene of miR-29b.CONCLUSION: miR-29b may be involved in the inhibition of HSC activation and migration, thereby inhibiting the process of liver fibrosis. The biological function of miR-29b may be through the direct targeting of TGF-β1, thus regulating and inhibiting the TGF-β/Smad signaling pathway.     

猪β防御素研究进展

β防御素是猪体内分泌的一类抗菌肽，广泛分布于各个组织中，在抵抗病原入侵和免疫调节中发挥着重要作用。猪β防御素对细菌、真菌和病毒都有很强的杀灭作用，并且具有分子量小、热稳定性好、无残留等优点，是理想的抗生素替代品。研究表明，猪β防御素的表达与个体抗病力正相关，增加内源性防御素表达或直接摄入外源性防御素均能增强机体免疫力并促进生长。脂肪酸、氨基酸、维生素、益生菌、病原微生物等外源刺激皆可影响β防御素的表达。随着防御素相关研究的深入，将有望解决养猪生产中长期使用抗生素而导致的耐药性、药物残留及环境污染等问题。文章介绍了猪β防御素的结构特征、表达特异性及生物学功能，主要综述了外源刺激对β防御素表达的影响及其作用机制，初步说明外源β防御素作为饲料添加剂在仔猪生产中的效果，旨在为猪β防御素的深入研究及其在生猪健康养殖中的应用提供参考。     

豫北山区“粮—草—羊”高效平衡养羊模式现状

从粮食作物、经济作物及饲草的种植,饲草料的加工调制与利用,全舍饲肉羊的养殖及杂交繁育,以及羊粪尿的资源化利用等方面介绍了豫北山区“粮—草—羊”高效平衡养羊模式发展现状,以期为促进豫北山区资源可持续利用、山区生态环境良性循环和社会经济可持续发展提供思路。     

Prevention and Controlling Effect of Intercropping on Pests and Diseases and Its Risk Control in Cotton Fields

Cotton is one of the major field crops which are seriously threatened by pests and diseases. In recent years, the ecological management of cotton pests and diseases with intercropping has become to be an interesting approach. Intercropping possibly increases the population of natural enemies and finally reduces the population density of pests as a result of changes in ecological structure and environmental conditions of farmland. Intercropping thus has been considered an important alternative in controlling pests and diseases. However, cotton field intercropping also has some limitations in controlling pests and diseases. Unreasonable intercropping system has many risks such as increasing labor input, increasing the difficulty of pests’ control, and aggravating pests and diseases. In this paper, the effects and the underlying mechanisms of cotton intercropping on cotton pests and diseases were reviewed. The possible risks and countermeasures used for ecological control of intercropping were also put forward, and the future and application of ecological management of cotton pests and diseases with intercropping were prospected.     

Assessing the utility of topographic variables in predicting structural complexity of tree stands in a reforested urban landscape

The transformation of natural landscapes into impervious built-up surfaces through urbanization is known to significantly interfere with the ecological integrity of urban landscapes and accelerate climate change and associated impacts. Although urban reforestation is widely recognised as an ideal mitigation practice against these impacts, it often has to compete with other lucrative land uses within an urban area. The often limited urban space provided for reforestation therefore necessitates the optimization of the ecological benefits, which demands spatially explicit information. The recent proliferation of tree stands structural complexity (SSC) and topographic data offer great potential for determining the ecological performance of reforested areas across an urban landscape. This study explores the potential of using topographic datasets to predict SSC in a reforested urban landscape and ranks the value of these topographic variables in determining SSC. Tree structural data from a reforested urban area was collected and fed into a tree stand structural complexity index, which was used to indicate ecological performance. Topographic variables (Topographic Wetness Index, slope, Area Solar Radiation and elevation)- were derived from a Digital Elevation Model (DEM) and used to predict SSC using the Partial Least Squares (PLS) regression technique. Results show that SSC varied significantly between the topographic variables. Results also show that the topographic variables could be used to reliably predict SSC. As expected, the Topographic Wetness Index and slope were the most important topographic determinants of SSC while elevation was the least valuable. These results provide valuable spatially explicit information about the ecological performance of the reforested areas within an urban landscape. Specifically, the study demonstrates the value of topographic data as aids to urban reforestation planning.     

PPARγ mediates effects of diosgenin on proliferation and apoptosis in human glioblastoma U87MG cells

AIM:To investigate the effect of diosgenin (Dio) on the proliferation, apoptosis and expression of peroxisome proliferator-activated receptor γ (PPARγ) in human glioblastoma U87MG cells and its possible mechanism. METHODS:Human astrocytes (HA) and U87MG cells were cultured in vitro and treated with Dio (0, 10, 20, 30, 40 and 50 μmol/L) and GW9662 (5 μmol/L) for 48 h, and then the cell viability was detected by CCK-8 assay. Cell colony formation assay was used to assess the proliferation potential. Flow cytometry was used to analyze the cell cycle distribution and apoptosis. The mRNA expression level of PPARγ was measured by RT-PCR. Western blot was used to determine the protein levels of PPARγ, cyclin D1, cyclin E1, Bcl-2 and Bax. RESULTS:Dio had no significant influence on the viabi-lity of HA (P>0.05). However, Dio remarkably reduced the viability of U87MG cells in a dose-dependent manner (P<0.05) with IC₅₀ of 24.31 μmol/L. Meanwhile, Dio remarkably diminished colony formation ability (P<0.05), induced G₀/G₁ phase arrest of the cell cycle and apoptosis (P<0.05), up-regulated the expression of PPARγ at mRNA and protein levels, increased the protein level of Bax (P<0.05), and down-regulated the protein levels of cyclin D1, cyclin E1 and Bcl-2 (P<0.05) in a dose-dependent manner. However, these effects induced by Dio were inhibited by GW9662 (P<0.05), a specific inhibitor of PPARγ. CONCLUSION:Dio may inhibit proliferation and induce apoptosis in human glioblastoma U87MG cells most likely via up-regulating the expression of PPARγ, and then down-regulating the protein levels of cyclin D1, cyclin E1 and Bcl-2, and up-regulating the protein level of Bax.     

伐地那非增加EGCG-β-乳球蛋白纳米粒对肝癌细胞的抑制作用

较高浓度的EGCG才能抑制癌细胞的增殖,通过纳米化和EGCG与其他药物的联合使用是提高EGCG生物活性的重要策略。本研究将EGCG和伐地那非（VD）同时包埋于β-乳球蛋白（β-Lg）纳米载体中,制备出EGCG-VD-β-Lg纳米粒（EVβ-NPs）,体外试验证实,EVβ-NPs能提高人肝癌细胞（HepG2细胞）中Caspase-3活性,使HepG2细胞在S期产生明显的阻滞,诱发细胞核分裂,从而导致HepG2细胞凋亡。研究结果表明,将EGCG与微量的VD联合使用,并通过纳米化包埋可以显著提高EGCG的抗癌活性。这一方法在EGCG抗癌制品的开发方面具有潜在的价值。